Comprehensive Analysis of the Association Between Human Non-obstructive Azoospermia and Plasticisers via Single-Cell and Traditional RNA Sequencing Methods

Abstract A father’s lifetime experience is a major risk factor for range of diseases in an individual. The influences exposure can also be transmitted to offspring. Previous studies demonstrated that plasticisers damage the male offspring reproductive system, but link between mammalian research and human non-obstructive azoospermia remains underexplored. Here, we analysed reproduction-related genes from four publicly available single-cell RNA-Seq datasets on GEO databases investigate correlation during pregnancy. R software was used statistical analyses. total 9 co-upregulated 1 co-downregulated gene were found. Gene Ontology enrichment analyses performed using “clusterProfiler” package. Single-cell conducted visualise expression pattern testis cell subgroups. Immunohistochemical images evaluate level protein testicular organs Human Protein Atlas. GSVA results provided further functional annotations. Three signature genes, i.e. COL1A1, CYP17A1 KIF11, might serve as promising diagnostic biomarkers caused by plasticisers. Our potential new protocol feasibility life or clinical intervention patients with azoospermia. We believe these observations will improve our understanding mechanisms plasticiser contributions help identify targets immunotherapy future.